DEPT The DEPT experiment is the preferred polarization transfer sequence for spectral editing. Using a set of three DEPT experiments, the Eft spectrometer is capable of distinguishing between quaternary, methyne, methylene and methyl groups in any number of compounds.   Below is a graph depicting the polarization transfer for the DEPT experiments. Notice that at 45 degrees, all of the lines are positive. In the DEPT-45 spectrum, one will see all carbons that have directly attached protons. At 90 degrees, CH2 and CH3 go through a null. Here, the spectrum will only show the CH carbons. Finally, at 135 degrees, CH and CH3 are positive while CH2 is negative. In all three experiments, quaternary carbon signals are quenched.